Details

Autor(en) / Beteiligte

• Cha, Min-Ji
• Jang, Jin-Kyung
• Ham, Onju
• Song, Byeong-Wook
• Lee, Se-Yeon
• Lee, Chang Yeon
• Park, Jun-Hee
• Lee, Jiyun
• Seo, Hyang-Hee
• Choi, Eunhyun
• Jeon, Woo-min
• Hwang, Hye Jin
• Shin, Hyun-Taek
• Choi, Eunmi
• Hwang, Ki-Chul

Titel

MicroRNA-145 suppresses ROS-induced Ca2+ overload of cardiomyocytes by targeting CaMKIIδ

Ist Teil von

• Biochemical and biophysical research communications, 2013-06, Vol.435 (4), p.720-726

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• •CaMKIIδ mediates H2O2-induced Ca2+ overload in cardiomyocytes.•miR-145 can inhibit Ca2+ overload.•A luciferase assay confirms that miR-145 functions as a CaMKIIδ-targeting miRNA.•Overexpression of miR-145 regulates CaMKIIδ-related genes and ameliorates apoptosis. A change in intracellular free calcium (Ca2+) is a common signaling mechanism of reperfusion-induced cardiomyocyte death. Calcium/calmodulin dependent protein kinase II (CaMKII) is a critical regulator of Ca2+ signaling and mediates signaling pathways responsible for functions in the heart including hypertrophy, apoptosis, arrhythmia, and heart disease. MicroRNAs (miRNA) are involved in the regulation of cell response, including survival, proliferation, apoptosis, and development. However, the roles of miRNAs in Ca2+-mediated apoptosis of cardiomyocytes are uncertain. Here, we determined the potential role of miRNA in the regulation of CaMKII dependent apoptosis and explored its underlying mechanism. To determine the potential roles of miRNAs in H2O2-mediated Ca2+ overload, we selected and tested 6 putative miRNAs that targeted CaMKIIδ, and showed that miR-145 represses CaMKIIδ protein expression and Ca2+ overload. We confirmed CaMKIIδ as a direct downstream target of miR-145. Furthermore, miR-145 regulates Ca2+-related signals and ameliorates apoptosis. This study demonstrates that miR-145 regulates reactive oxygen species (ROS)-induced Ca2+ overload in cardiomyocytes. Thus, miR-145 affects ROS-mediated gene regulation and cellular injury responses.