Details

Autor(en) / Beteiligte

• Huang, Qiansheng
• Vera Delgado, Juan Manuel
• Seni Pinoargote, Oscar David
• Llaguno, Ricardo Avellán

Titel

Molecular evolution of the Slc15 family and its response to waterborne copper and mercury exposure in tilapia

Ist Teil von

• Aquatic toxicology, 2015-06, Vol.163, p.140-147

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• •The molecular evolution of Slc15 family wasstudied across eukaryotes.•Tissue expression profiles were compared in tilapia.•Temporal expressions were revealed during fasting–refeeding process.•The recovery was impaired by environmental concentration of copper. The solute carrier 15 family (Slc15), also called oligopeptide transporter family (Pept), was well-known for its role in the cellular uptake of di/tripeptides and peptide-like molecules. Our understanding of Slc15 family has already been enlarged since the rapid increasing of genome information; however, efforts are still expected to reveal the diversification of the family in an evolutionary manner. In the study, the sequence information were collected and analyzed through eleven eukaryotic organism representatives, especially in fish species. Gene expansion was observed through the evolution of the family. Further study was carried out with the representative species-Nile tilapia (Oreochromis niloticus). Tissue expression profiles were compared among members of the Slc15 family. Generally, they were all highly expressed both in the intestine and stomach, however, different members possessed its special tissue expression pattern. The mRNA levels of all the members (except Slc15a4) decreased after fasting while refeeding could restore the expression level. The recovery ability was impaired after exposure to environmental relevant concentration of copper (Cu2+, 160nmol/L). By contrast, mercury (Hg2+, 25nmol/L) did not exert significant impact on the recovery ability.