Details

Autor(en) / Beteiligte

• Tessaro, Irene
• Modina, Silvia C.
• Crotti, Gabriella
• Franciosi, Federica
• Colleoni, Silvia
• Lodde, Valentina
• Galli, Cesare
• Lazzari, Giovanna
• Luciano, Alberto M.

Titel

Transferability and inter-laboratory variability assessment of the in vitro bovine oocyte fertilization test

Ist Teil von

• Reproductive toxicology (Elmsford, N.Y.), 2015-01, Vol.51, p.106-113

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •Bovine in vitro fertilization assay (bIVF) can be effectively transferred to a second laboratory.•Bovine in vitro fertilization assay can advance toward the validation process.•The combination of bIVF with oocyte maturation test (bIVM) become part of an integrated testing strategy.•bIVM and bIVF are alternative methods suitable to predict chemical hazards on mammalian fertility. The dramatic increase in the number of animals required for reproductive toxicity testing imposes the validation of alternative methods to reduce the use of laboratory animals. As we previously demonstrated for in vitro maturation test of bovine oocytes, the present study describes the transferability assessment and the inter-laboratory variability of an in vitro test able to identify chemical effects during the process of bovine oocyte fertilization. Eight chemicals with well-known toxic properties (benzo[a]pyrene, busulfan, cadmium chloride, cycloheximide, diethylstilbestrol, ketoconazole, methylacetoacetate, mifepristone/RU-486) were tested in two well-trained laboratories. The statistical analysis demonstrated no differences in the EC50 values for each chemical in within (inter-runs) and in between-laboratory variability of the proposed test. We therefore conclude that the bovine in vitro fertilization test could advance toward the validation process as alternative in vitro method and become part of an integrated testing strategy in order to predict chemical hazards on mammalian fertility.