Hormones and behavior, 2015-05, Vol.71, p.41-48
2015
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- Autor(en) / Beteiligte
- Titel
- Effects of uterine and lactational exposure to di-(2-ethylhexyl) phthalate on spatial memory and NMDA receptor of hippocampus in mice
- Ist Teil von
- Hormones and behavior, 2015-05, Vol.71, p.41-48
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Di-(2-ethylhexyl) phthalate (DEHP) is an environmental endocrine disrupter. Currently, little is known about neurodevelopmental toxicity of DEHP in wildlife and humans. The present study investigated the effects of DEHP, focusing on the changes in the behavior of offspring mice at the ages of 6 and 12w, respectively, following utero and lactational exposure to DEHP (10, 50, and 200mg/kg/d) from gestation day 7 through postnatal day 21. The results of open field tasks showed that DEHP increased the grooming of males at age 6w and females at age 12w but decreased the frequency of rearing of 6-w-old females and the number of grid crossings of 12-w-old females. In the Morris water maze task, 50 and 200mg/kg/d DEHP significantly prolonged the time of searching the hidden platform in water maze and reduced the time staying in the target quadrant during a probe trial of 6-w-old male mice, but not of 6-w-old females nor 12-w-old mice of both sexes, suggesting an impaired spatial learning and memory among younger males after perinatal exposure to DEHP. Western blot analyses further showed that DEHP at 50 and 200mg/kg/d decreased the levels of the N-methyl-d-aspartic acid (NMDA) receptor subunits NR1 and NR2B in the hippocampus of 6-w-old males. These results suggest that uterine and lactational exposure to low doses of DEHP sex-specifically impacted behaviors, including locomotion activity and spatial memory, via the concomitant inhibition of the NMDA receptor of the hippocampus in offspring mice. •DEHP increased grooming of 6-w old male mice and 12-w-old females in open field.•DEHP decreased rearing and grid crossings of female mice in open field.•DEHP prolonged latency of locating the platform in Morris water maze of 6-w-old males.•DEHP reduced the time staying in the target quadrant in water maze of 6-w-old males.•DEHP reduced NMDA receptor subunit NR2B in hippocampus of 6-w-old males.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0018-506XeISSN: 1095-6867DOI: 10.1016/j.yhbeh.2015.03.008Titel-ID: cdi_proquest_miscellaneous_1732811275
- Format
- –
- Schlagworte
- Animal behavior, Animal memory, Animals, Behavior, Body Weight - drug effects, Di-(2-ethylhexyl) phthalate, Diethylhexyl Phthalate - pharmacology, Endocrine Disruptors - pharmacology, Endocrine system, Female, Hippocampus - drug effects, Hippocampus - metabolism, Lactation, Male, Maze Learning, Mice, Mice, Inbred ICR, Motor Activity - drug effects, NMDA receptor, Organ Size - drug effects, Pregnancy, Prenatal Exposure Delayed Effects - psychology, Receptors, N-Methyl-D-Aspartate - drug effects, Receptors, N-Methyl-D-Aspartate - metabolism, Rodents, Spatial learning and memory, Spatial Memory - drug effects