Proceedings of the National Academy of Sciences - PNAS, 2015-09, Vol.112 (36), p.11353-11358
2015
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- Autor(en) / Beteiligte
- Titel
- Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2015-09, Vol.112 (36), p.11353-11358
- Ort / Verlag
- United States: National Acad Sciences
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Biofilm formation is a complex, ordered process. In the opportunistic pathogen Pseudomonas aeruginosa, Psl and Pel exopolysaccharides and extracellular DNA (eDNA) serve as structural components of the biofilm matrix. Despite intensive study, Pel's chemical structure and spatial localization within mature biofilms remain unknown. Using specialized carbohydrate chemical analyses, we unexpectedly found that Pel is a positively charged exopolysaccharide composed of partially acetylated 1→4 glycosidic linkages of N-acetylgalactosamine and N-acetylglucosamine. Guided by the knowledge of Pel's sugar composition, we developed a tool for the direct visualization of Pel in biofilms by combining Pel-specific Wisteria floribunda lectin staining with confocal microscopy. The results indicate that Pel cross-links eDNA in the biofilm stalk via ionic interactions. Our data demonstrate that the cationic charge of Pel is distinct from that of other known P. aeruginosa exopolysaccharides and is instrumental in its ability to interact with other key biofilm matrix components.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1503058112Titel-ID: cdi_proquest_miscellaneous_1727678774
- Format
- –
- Schlagworte
- Bacteria, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Biofilms, Biological Sciences, Carbohydrates, Cations - chemistry, Deoxyribonucleic acid, DNA, DNA, Bacterial - chemistry, DNA, Bacterial - genetics, DNA, Bacterial - metabolism, Extracellular Matrix - metabolism, Extracellular Space - genetics, Glycosyltransferases - genetics, Glycosyltransferases - metabolism, Immunoblotting, Microscopy, Confocal, Mutation, Plant Lectins - chemistry, Plant Lectins - metabolism, Polysaccharides, Bacterial - chemistry, Polysaccharides, Bacterial - metabolism, Pseudomonas aeruginosa, Pseudomonas aeruginosa - genetics, Pseudomonas aeruginosa - metabolism, Pseudomonas aeruginosa - physiology, Receptors, N-Acetylglucosamine - chemistry, Receptors, N-Acetylglucosamine - metabolism, Staining and Labeling - methods, Wisteria floribunda