Details

Autor(en) / Beteiligte

• de Sousa, Aline A.
• dos Reis, Renata R.
• de Lima, Camila M.
• de Oliveira, Marcus A.
• Fernandes, Taiany N.
• Gomes, Giovanni F.
• Diniz, Daniel G.
• Magalhães, Nara M.
• Diniz, Cristovam G.
• Sosthenes, Marcia C. K.
• Bento-Torres, João
• Diniz Jr, José Antonio P.
• Vasconcelos, Pedro F. da C.
• Diniz, Cristovam Wanderley P.

Titel

Three-dimensional morphometric analysis of microglial changes in a mouse model of virus encephalitis: age and environmental influences

Ist Teil von

• The European journal of neuroscience, 2015-08, Vol.42 (4), p.2036-2050

Ort / Verlag

France: Blackwell Publishing Ltd

Erscheinungsjahr

2015

Quelle

EBSCOhost Psychology and Behavioral Sciences Collection

Beschreibungen/Notizen

• Many RNA virus CNS infections cause neurological disease. Because Piry virus has a limited human pathogenicity and exercise reduces activation of microglia in aged mice, possible influences of environment and aging on microglial morphology and behavior in mice sublethal encephalitis were investigated. Female albino Swiss mice were raised either in standard (S) or in enriched (EE) cages from age 2 to 6 months (young – Y), or from 2 to 16 months (aged – A). After behavioral tests, mice nostrils were instilled with Piry‐virus‐infected or with normal brain homogenates. Brain sections were immunolabeled for virus antigens or microglia at 8 days post‐infection (dpi), when behavioral changes became apparent, and at 20 and 40 dpi, after additional behavioral testing. Young infected mice from standard (SYPy) and enriched (EYPy) groups showed similar transient impairment in burrowing activity and olfactory discrimination, whereas aged infected mice from both environments (EAPy, SAPy) showed permanent reduction in both tasks. The beneficial effects of an enriched environment were smaller in aged than in young mice. Six‐hundred and forty microglial cells, 80 from each group were reconstructed. An unbiased, stereological sampling approach and multivariate statistical analysis were used to search for microglial morphological families. This procedure allowed distinguishing between microglial morphology of infected and control subjects. More severe virus‐associated microglial changes were observed in young than in aged mice, and EYPy seem to recover microglial homeostatic morphology earlier than SYPy . Because Piry‐virus encephalitis outcomes were more severe in aged mice, it is suggested that the reduced inflammatory response in those individuals may aggravate encephalitis outcomes. We measured the influences of aging and environment on behavioral and microglial changes in a mice model of virus sublethal encephalitis. We found that aged infected mice, showed permanent behavioral impairments and this was associated with smaller morphological changes in CA3 microglia. Young infected mice showed significant CA3 microglial changes with transitory or absent behavioral impairments. Reduced inflammatory response in immunosenescent individuals may aggravate encephalitis outcomes.