Details

Autor(en) / Beteiligte

• Mirto, H
• Hengé-Napoli, M.H
• Gibert, R
• Ansoborlo, E
• Fournier, M
• Cambar, J

Titel

Intracellular behaviour of uranium(VI) on renal epithelial cell in culture (LLC-PK 1): influence of uranium speciation

Ist Teil von

• Toxicology letters, 1999-02, Vol.104 (3), p.249-256

Ort / Verlag

Shannon: Elsevier Ireland Ltd

Erscheinungsjahr

1999

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The main objective of this work was to assess the potentiality of in vitro models to study and understand the uranium-induced cytotoxicity on renal cells. Cytotoxicity and morphological studies were performed in a tubular proximal original established cell line (LLC-PK 1 cell line). Dose-dependent cytotoxicity response was obtained with the uranium–bicarbonate complex. In vitro experiments revealed a toxicity of uranium–bicarbonate complexes after a 24-h exposition and for concentrations ranging from 7×10 −4 M to 10 −3 M. In contrast, a lack of cytotoxicity of uranium(VI)–citrate complexes studied using the same experimental conditions was noticed. Furthermore, electron transmission microscopy and X-ray microanalysis studies, after exposition of LLC-PK 1 cells to the uranium-bicarbonate system ([U]=8×10 −4 M) revealed that uranium entered into the cells and it was precipitated within the cytoplasmic compartment as uranyl phosphate needles. Similar morphological studies conducted with citrate complexes did not show any intake of uranium by LLC-PK 1 cells. Experiments conducted in phosphate free culture medium showed that uranium was incorporated as a soluble material and that the association of the metal with phosphate ions occurred in the cytoplasmic compartment of LLC-PK 1 cells.