FEMS microbiology letters, 1999-04, Vol.173 (2), p.303-310
1999
Details
- Autor(en) / Beteiligte
- Titel
- Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins
- Ist Teil von
- FEMS microbiology letters, 1999-04, Vol.173 (2), p.303-310
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1999
- Link zum Volltext
- Quelle
- Wiley HSS Collection
- Beschreibungen/Notizen
- Abstract The regulation of nitrogen assimilation was investigated in the Gram-positive actinomycete Corynebacterium glutamicum. Biochemical studies and site-directed mutagenesis revealed that glutamine synthetase activity is regulated via adenylylation in this organism. The genes encoding the central signal transduction protein PII (glnB) and the primary nitrogen sensor uridylyltransferase (glnD) were isolated and sequenced. Additionally, genes putatively involved in the degradation of ornithine (ocd) and sarcosine (soxA), ammonium uptake (amtP) and protein secretion (ftsY, srp) were identified in C. glutamicum. Based on these observations, the mechanism of N regulation in C. glutamicum is similar to that of the Gram-negative Escherichia coli. As deduced from data base searches, the described regulation may also hold true for the important pathogen Mycobacterium glutamicum.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1097eISSN: 1574-6968DOI: 10.1111/j.1574-6968.1999.tb13518.xTitel-ID: cdi_proquest_miscellaneous_17196307
- Format
- –
- Schlagworte
- Actinomycete, Ammonium, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Bacteriology, Biodegradation, Biological and medical sciences, Corynebacterium - enzymology, Corynebacterium - genetics, Corynebacterium - growth & development, Corynebacterium glutamicum, Data search, E coli, Escherichia coli, Fundamental and applied biological sciences. Psychology, Gene expression, Gene Expression Regulation, Bacterial, Genes, Genes, Bacterial, Genetics, Glutamate-ammonia ligase, Glutamate-Ammonia Ligase - genetics, Glutamine, Gram‐positive bacterium, Microbiology, Molecular Sequence Data, Mycobacterium, Mycobacterium glutamicum, Nitrogen, Nitrogen - metabolism, Nitrogen control, Nucleotidyltransferases - genetics, Ornithine, Ornithine - metabolism, PII Nitrogen Regulatory Proteins, Plasmids - genetics, Proteins, Quaternary Ammonium Compounds - metabolism, Regulations, Sarcosine, Sarcosine - metabolism, Secretion, Signal transduction, Site-directed mutagenesis