Details

Autor(en) / Beteiligte

• Jiang, Jun
• Zhang, Dapeng
• Walker, Steven
• Gu, Chenglin
• Ke, Ya
• Yung, Wing Ho
• Chen, Shih-chi

Titel

Fast 3-D temporal focusing microscopy using an electrically tunable lens

Ist Teil von

• Optics express, 2015-09, Vol.23 (19), p.24362-24368

Ort / Verlag

United States

Erscheinungsjahr

2015

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In this paper, we present a 3-D temporal focusing microscope based on an electrically tunable lens (ETL) and a femtosecond regenerative laser amplifier. The focus-tunable lens provides a fast and compact way to perform non-mechanical z-scanning and resolves the blurry image issue compared with GVD-based z-scanning methods. The optical performance of the temporal focusing system, including z-scanning characteristics, the associated the magnification variation, and the lateral and axial resolution, has been studied and characterized using calibrated Rhodamine-6G thin film sample, fluorescent beads, and pollen samples. Lastly, we demonstrate the optical cross-sectioning and z-scanning capability with an in vivo experiment, where Ca(2+) imaging of neurons in GaCamp6 labeled zebrafish was performed.