Details

Autor(en) / Beteiligte

• Li, Xi-Ling
• Li, Gao
• Jiang, Ying-Zi
• Kang, Dongzhou
• Jin, Cheng Hua
• Shi, Qing
• Jin, Toufeng
• Inoue, Koichi
• Todoroki, Kenichiro
• Toyo'oka, Toshimasa
• Min, Jun Zhe

Titel

Human nails metabolite analysis: A rapid and simple method for quantification of uric acid in human fingernail by high-performance liquid chromatography with UV-detection

Ist Teil von

• Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2015-10, Vol.1002, p.394-398

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •We have developed a sensitive, accurate and simple HPLC-UV method for the quantification of UA in human fingernails.•This determination of UA in the fingernail of healthy men and women volunteers by HPLC-UV is the first reported example.•As for the concentration of each fingernail, the forefinger, middle finger and third finger are relatively stable. A rapid and simple analytical method for the quantification of uric acid (UA) in human fingernails by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection is described. UA was extracted from human fingernail samples at 90°C for 20min, then separated on an Inertsil ODS-2 column (250×4.6mm I.D., 5.0μm, GL Sciences) by isocratic elution using methanol: 74mM phosphate buffer (pH 2.2) 2:98 (v/v). An UV detector was used to monitor at 284nm. The results indicated that under optimized measurement conditions results were achieved within 8.0min, and a good linearity was achieved from the calibration curves (r2>0.9999) in the range of 1.0–10000ng; the limit of detection (S/N=3) was 2.0pg, the inter-day and intra-day assay precisions were all less than 0.46% and the mean recoveries (%) of the uric acid spiked in the human fingernail were 101.95%. The amounts of UA in the fingernails of healthy volunteers were determined.