Neuroscience letters, 1998-11, Vol.257 (2), p.116-118
1998
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Antiphospholipid antibodies bind to rat cerebellar granule cells: the role of N-methyl- d-aspartate receptors
- Ist Teil von
- Neuroscience letters, 1998-11, Vol.257 (2), p.116-118
- Ort / Verlag
- Shannon: Elsevier Ireland Ltd
- Erscheinungsjahr
- 1998
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- IgGs from sera containing antiphospholipid antibodies (aPL), detected as antibodies to cardiolipin, or control sera were incubated with rat cerebellar granule cells in primary culture. Using a mitochondrial dehydrogenase activity assay (MTT test), aPL IgGs were shown to decrease MTT metabolism after 24 h incubation with the cells, and to cause non-toxic amounts of glutamate to become neurotoxic when added to the cells for 45 min. Acute and chronic aPL toxicity were prevented by MK-801. Sera containing aPL bound to intact cerebellar neurons, as revealed by an immunofluorescent technique. These results suggest that antiphospholipid antibodies interfere with excitatory pathways in glutamatergic cerebellar granule cells by a mechanism involving overactivation of the NMDA glutamate receptor.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0304-3940eISSN: 1872-7972DOI: 10.1016/S0304-3940(98)00793-9Titel-ID: cdi_proquest_miscellaneous_17152148
- Format
- –
- Schlagworte
- Animals, Antibodies, Antiphospholipid - immunology, Antiphospholipid antibodies, Biological and medical sciences, Central nervous system, Central neurotransmission. Neuromudulation. Pathways and receptors, Cerebellum - cytology, Cerebellum - immunology, Coloring Agents - metabolism, Dizocilpine Maleate - pharmacology, Excitatory Amino Acid Antagonists - pharmacology, Excitotoxicity, Fundamental and applied biological sciences. Psychology, Glutamate, Glutamic Acid - pharmacology, Humans, Immunoglobulin G - immunology, Mitochondria - enzymology, N-methyl- d-aspartate receptor, Neurons - immunology, Neurotoxins - pharmacology, Oxidation-Reduction, Oxidoreductases - metabolism, Rat cerebellar granules, Rats, Receptors, N-Methyl-D-Aspartate - physiology, Tetrazolium Salts - metabolism, Thiazoles - metabolism, Vertebrates: nervous system and sense organs