Details

Autor(en) / Beteiligte

• Zhao, X.
• Shi, F.
• Zhan, W.

Titel

Overexpression of ZWF1 and POS5 improves carotenoid biosynthesis in recombinant Saccharomyces cerevisiae

Ist Teil von

• Letters in applied microbiology, 2015-10, Vol.61 (4), p.354-360

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2015

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• Recombinant Saccharomyces cerevisiae expressing exogenous carotenogenic genes can synthesize carotenoids. NADPH is a key cofactor for carotenoid biosynthesis, while glucose‐6‐phosphate dehydrogenase (Zwf1) and an NADH kinase (Pos5) are the two main NADPH‐supplying sources in S. cerevisiae. Here, the effect of ZWF1 and POS5 overexpression on carotenoid yield in recombinant S. cerevisiae was explored. The initial carotenogenic strain Sc‐EYBIH+I which expressed crtE, crtYB, crtI, cHMG1 and another copy of crtI could synthesize 1·35 ± 0·13 mg l−1 of lycopene and 0·32 ± 0·02 mg l−1 of β‐carotene. When ZWF1 was overexpressed (Sc‐EYBIZH+I), glucose‐6‐phosphate dehydrogenase activity increased by 103‐fold, the transcription level of crtE and crtI increased significantly, the lycopene and β‐carotene yield increased to 2·29 ± 0·06 and 0·38 ± 0·02 mg l−1 respectively. When POS5 was overexpressed (Sc‐EYBIPH+I), NAD kinase activity increased by 5·5‐fold, the transcription level of crtE, crtYB and crtI increased obviously, the lycopene and β‐carotene yield increased to 2·50 ± 0·11 and 0·53 ± 0·03 mg l−1 respectively. Therefore, improvement of NADPH supply contributed to carotenoids biosynthesis in S. cerevisiae and overexpression of POS5 was more effective than overexpression of ZWF1. This study provides a new strategy for enhancing carotenoid biosynthesis. Significance and Impact of the Study NADPH is a key cofactor for carotenoid biosynthesis. Glucose‐6‐phosphate dehydrogenase (Zwf1) and an NADH kinase (Pos5) are effective NADPH‐supplying sources in Saccharomyces cerevisiae. When ZWF1 and POS5 were overexpressed in a carotenoid‐producing S. cerevisiae strain individually, the total yield of lycopene and β‐carotene increased by 59·9% and 81·4%, respectively, and the final product β‐carotene yield increased by 18·8% and 65·6% respectively. This suggests the improvement of NADPH supply as a useful strategy for carotenoids biosynthesis. Significance and Impact of the Study: NADPH is a key cofactor for carotenoid biosynthesis. Glucose‐6‐phosphate dehydrogenase (Zwf1) and an NADH kinase (Pos5) are effective NADPH‐supplying sources in Saccharomyces cerevisiae. When ZWF1 and POS5 were overexpressed in a carotenoid‐producing S. cerevisiae strain individually, the total yield of lycopene and β‐carotene increased by 59·9% and 81·4%, respectively, and the final product β‐carotene yield increased by 18·8% and 65·6% respectively. This suggests the improvement of NADPH supply as a useful strategy for carotenoids biosynthesis.