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•Chlorella sp. (FACHB-1748) could utilize urea as nitrogen source.•Chlorella sp. could be cultured in 70L bioreactor with natural sunlight under outdoor conditions.•The lipid productivity could be improved under the condition of sodium chloride or acetate.•The quality properties of the biodiesel met the standard of National Petroleum Agency (ANP255).
Chlorella sp. (FACHB-1748) was cultivated outdoors under natural sunlight to evaluate its potential for biofuel production. Urea was selected as nitrogen source, and the concentration was optimized. When the culture reached the late exponential stage, a triggering lipid accumulation test was conducted using different concentrations of sodium chloride and acetate. A scaling-up experiment was also conducted in a 70L photobioreactor. The highest biomass productivity (222.42, 154.48mg/L/d) and lipid productivity (64.30, 33.69mg/L/d) were obtained with 0.1g/L urea in 5 and 70L bioreactors, respectively. The highest lipid content (43.25%) and lipid yield (1243.98mg/L) were acquired with the combination of 10g/L sodium chloride and acetate. Moreover, the qualities of biodiesel, cetane number, saponification value, iodine value, and cold filter plugging point complied with the standards set by the National Petroleum Agency (ANP255), Standard ASTMD6751, and European Standard (EN 14214).