Details

Autor(en) / Beteiligte

• Buescher, Joerg M
• Antoniewicz, Maciek R
• Boros, Laszlo G
• Burgess, Shawn C
• Brunengraber, Henri
• Clish, Clary B
• DeBerardinis, Ralph J
• Feron, Olivier
• Frezza, Christian
• Ghesquiere, Bart
• Gottlieb, Eyal
• Hiller, Karsten
• Jones, Russell G
• Kamphorst, Jurre J
• Kibbey, Richard G
• Kimmelman, Alec C
• Locasale, Jason W
• Lunt, Sophia Y
• Maddocks, Oliver D K
• Malloy, Craig
• Metallo, Christian M
• Meuillet, Emmanuelle J
• Munger, Joshua
• Nöh, Katharina
• Rabinowitz, Joshua D
• Ralser, Markus
• Sauer, Uwe
• Stephanopoulos, Gregory
• St-Pierre, Julie
• Tennant, Daniel A
• Wittmann, Christoph
• Vander Heiden, Matthew G
• Vazquez, Alexei
• Vousden, Karen
• Young, Jamey D
• Zamboni, Nicola
• Fendt, Sarah-Maria

Titel

A roadmap for interpreting (13)C metabolite labeling patterns from cells

Ist Teil von

• Current opinion in biotechnology, 2015-08, Vol.34, p.189-201

Ort / Verlag

England

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Measuring intracellular metabolism has increasingly led to important insights in biomedical research. (13)C tracer analysis, although less information-rich than quantitative (13)C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting (13)C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.