Details

Autor(en) / Beteiligte

• Kellmann, J.W. (Max-Planck-Institut fuer Zuechtungsforschung, Koeln (Germany))
• Kleinow, T
• Engelhardt, K
• Philipp, C
• Wegener, D
• Schell, J
• Schreier, P.H

Titel

Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants

Ist Teil von

• Plant molecular biology, 1996-01, Vol.30 (2), p.351-358

Ort / Verlag

Netherlands

Erscheinungsjahr

1996

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;1 and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea. The second gene, A.h.Chi2;1, was only expressed after treatment with the fungal spores. Transgenic tobacco plants containing the complete peanut A.h.Chi2;1 gene exhibited essentially the same expression pattern in leaves as observed in peanut cell cultures. Expression characteristics of transgenic tobacco carrying a promoter-GUS fusion of A.h.Chi2;1 are described.