Details

Autor(en) / Beteiligte

• Rupprecht, Kevin R.
• Lang, Ewa Z.
• Gregory, Svetoslava D.
• Bergsma, Janet M.
• Rae, Tracey D.
• Fishpaugh, Jeffrey R.

Titel

A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration

Ist Teil von

• Analytical biochemistry, 2015-10, Vol.486, p.78-80

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use with protein solutions where SDS concentration is a critical factor, so we modified this method after examining multiple parameters (solvent, pH, buffers, and light exposure). The improved method is simple to implement, robust, accurate, and (most important) precise.