Details

Autor(en) / Beteiligte

• Porras-Domínguez, Jaime R.
• Ávila-Fernández, Ángela
• Miranda-Molina, Afonso
• Rodríguez-Alegría, María Elena
• Munguía, Agustín López

Titel

Bacillus subtilis 168 levansucrase (SacB) activity affects average levan molecular weight

Ist Teil von

• Carbohydrate polymers, 2015-11, Vol.132, p.338-344

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• •Levansucrase activity defines levan molecular weight distribution in synthesis with sucrose.•High levansucrase activity results in the synthesis of low molecular weight levan.•Low levansucrase activity results in the synthesis of high molecular weight levan.•Enzyme concentration affects hydrolysis and transferase initial reaction ratio. Levan is a fructan polymer that offers a variety of applications in the chemical, health, cosmetic and food industries. Most of the levan applications depend on levan molecular weight, which in turn depends on the source of the synthesizing enzyme and/or on reaction conditions. Here we demonstrate that in the particular case of levansucrase from Bacillus subtilis 168, enzyme concentration is also a factor defining the molecular weight levan distribution. While a bimodal distribution has been reported at the usual enzyme concentrations (1U/ml equivalent to 0.1μM levansucrase) we found that a low molecular weight normal distribution is solely obtained al high enzyme concentrations (>5U/ml equivalent to 0.5μM levansucrase) while a high normal molecular weight distribution is synthesized at low enzyme doses (0.1U/ml equivalent to 0.01μM of levansucrase).