Molecular plant, 2015-08, Vol.8 (8), p.1253-1273
2015
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- Autor(en) / Beteiligte
- Titel
- High REDOX RESPONSIVE TRANSCRIPTION FACTOR1 Levels Result in Accumulation of Reactive Oxygen Species in Arabidopsis thaliana Shoots and Roots
- Ist Teil von
- Molecular plant, 2015-08, Vol.8 (8), p.1253-1273
- Ort / Verlag
- England: Elsevier Inc
- Erscheinungsjahr
- 2015
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Redox Responsive Transcription Factor1 （RRTF1） in Arabidopsis is rapidly and transiently upregulated by H202, as well as biotic- and abiotic-induced redox signals. RRTF1 is highly conserved in angio- sperms, but its physiological role remains elusive. Here we show that inactivation of RRTF1 restricts and overexpression promotes reactive oxygen species （ROS） accumulation in response to stress. Transgenic lines overexpressing RRTF1 are impaired in root and shoot development, light sensitive, and susceptible to Alternaria brassicae infection. These symptoms are diminished by the beneficial root endophyte Piriformospora indica, which reduces ROS accumulation locally in roots and systemi- cally in shoots, and by antioxidants and ROS inhibitors that scavenge ROS. More than 800 genes were detected in mature leaves and seedlings of transgenic lines overexpressing RRTF1; ∽40% of them have stress-, redox-, ROS-regulated-, ROS-scavenging-, defense-, cell death- and related functions. Bioinformatic analyses and in vitro DNA binding assays demonstrate that RRTF1 binds to GCC-box-like sequences in the promoter of RRTFl-responsive genes. Upregulation of RRTF1 by stress stimuli and H202 requires WRKY18/40/60. RRTF1 is co-regulated with the phylogenet- ically related RAP2.6, which contains a GCC-box-like sequence in its promoter, but transgenic lines overexpressing RAP2.6 do not accumulate higher ROS levels. RRTF1 also stimulates systemic ROS accumulation in distal non-stressed leaves. We conclude that the elevated levels of the highly conserved RRTF1 induce ROS accumulation in response to ROS and ROS-producing abiotic and biotic stress signals.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1674-2052eISSN: 1752-9867DOI: 10.1016/j.molp.2015.03.011Titel-ID: cdi_proquest_miscellaneous_1702086364
- Format
- –
- Schlagworte
- abiotic and biotic stress, Acetylcysteine - pharmacology, Alternaria - physiology, Arabidopsis - metabolism, Arabidopsis - microbiology, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Base Sequence, Cell Death - drug effects, Ditiocarb - pharmacology, Gene Expression Regulation, Plant - drug effects, Genes, Plant, H2O2, Light, Molecular Sequence Data, Pathogen-Associated Molecular Pattern Molecules - metabolism, Phenotype, Plant Diseases - genetics, Plant Diseases - microbiology, Plant Roots - drug effects, Plant Roots - genetics, Plant Roots - metabolism, Plant Roots - radiation effects, Plant Shoots - drug effects, Plant Shoots - metabolism, Plant Shoots - radiation effects, Plants, Genetically Modified, Promoter Regions, Genetic - genetics, RAP2.6, reactive oxygen species, Reactive Oxygen Species - metabolism, REDOX RESPONSIVE TRANSCRIPTION FACTOR1, RRTF1 promoter binding sites, Seedlings - drug effects, Seedlings - genetics, Seedlings - microbiology, Seedlings - radiation effects, Stress, Physiological - drug effects, Stress, Physiological - genetics, Stress, Physiological - radiation effects, Transcription Factors - genetics, Transcription Factors - metabolism, 启动子序列, 应力积累, 拟南芥, 氧化还原反应, 水, 活性氧, 生物诱导, 转录因子