Details

Autor(en) / Beteiligte

• Sadowski, P D

Titel

The Flp recombinase of the 2- mu m plasmid of Saccharomyces cerevisiae

Ist Teil von

• Progress in nucleic acid research and molecular biology, 1995-01, Vol.51, p.53-92

Erscheinungsjahr

1995

Beschreibungen/Notizen

• Most strains of the yeast, Saccharomyces cerevisiae, harbor about 100 copies of an autonomously replicating plasmid, the 2- mu m plasmid. Although the plasmid confers no advantage to its host, and in fact confers a slight disadvantage, it is very stable. Its rate of loss is similar to 7.6 x 10 super(-5) per cell per generation. Because of its high copy-number and stability, the 2- mu m plasmid has been a useful model for studying DNA replication, recombination, regulation of gene expression, and plasmid segregation in eukaryotic organisms. The plasmid has also served as the basis of many important autonomously replicating high-copy expression vectors in yeast. One of the plasmid's gene products, the Flp protein, is the subject of this review. Flp is the first eukaryotic, conservative site-specific recombinase to be subjected to detailed study.