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We studied the influence of six edaphic biostimulants/biofertilizers (BSs) manufactured by the pH-stat method from different sewage sludge (SS): SS1 (an anaerobic mature sludge, one year old), SS2 (an aerobic young sludge, without maturation) and SS3 (an aerobic mature sludge, four months old), not previously autoclaved (A) and autoclaved (B), by analysing their effects on soil biological properties. Soil enzymatic activities were measured at 1, 3, 5, 7, 15, 30 and 60 days of the incubation period, whereas the 16S rDNA-DGGE profiles were determined at 0, 5 and 60 days. The enzymatic activities were significantly stimulated. The highest stimulation was found in the B2 treatment followed by B3, A2, A3, B1 and A1 treatments. Increasing the number of lower molecular weight proteins in the BS enhances the stimulation of soil enzymatic activities. The application of BS caused at 5 days of the incubation period temporal variations in the soil bacterial community structure.