Angewandte Chemie International Edition, 2014-02, Vol.53 (8), p.2198-2202
International ed. in English, 2014
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Irreversible Site-Specific Hydrazinolysis of Proteins by Use of Sortase
- Ist Teil von
- Angewandte Chemie International Edition, 2014-02, Vol.53 (8), p.2198-2202
- Auflage
- International ed. in English
- Ort / Verlag
- Weinheim: WILEY-VCH Verlag
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Sortase‐mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C‐terminal modification of proteins with functional units as well as for the preparation of C‐to‐C fusion proteins. Takes all sort(ase)s: Sortase A mediated hydrazinolysis reactions of proteins with hydrazine or its derivatives were developed as an efficient process for the production of recombinant protein hydrazides. This reaction provides an alternative approach for the semichemical synthesis as well as C‐terminal modification of proteins.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1433-7851eISSN: 1521-3773DOI: 10.1002/anie.201310010Titel-ID: cdi_proquest_miscellaneous_1701008095
- Format
- –
- Schlagworte
- Amino Acid Sequence, Aminoacyltransferases - metabolism, Bacterial Proteins - metabolism, Bacteriology, Cysteine Endopeptidases - metabolism, Derivatives, Fluorescein - chemistry, Hydrazides, Hydrazine, Hydrazines, Hydrazines - chemistry, hydrazones, Hydrolysis, ligation, Peptides - chemistry, Peptides - metabolism, protein modification, Protein Structure, Tertiary, Proteins, Recombinant, Recombinant Proteins - chemistry, Recombinant Proteins - metabolism, Semisynthesis, Single-Domain Antibodies - chemistry, Single-Domain Antibodies - immunology, Sortase, Synthesis, Thioesters