Details

Autor(en) / Beteiligte

• Rosenstein, Barry S.
• Vaslet, Charles A.

Titel

Molecular cloning of the human gene SUVCC2 associated with mutagenesis following the induction of non-dimer DNA damages by solar UV radiation

Ist Teil von

• Journal of photochemistry and photobiology. B, Biology, 1995-06, Vol.28 (3), p.203-211

Ort / Verlag

Switzerland: Elsevier B.V

Erscheinungsjahr

1995

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• A mutant cell line, DRP 512, sensitive to the induction of non-dimer DNA damages produced by solar UV radiation was derived from ICR 2A frog cells. In addition, the DRP 512 cells exhibited an abnormally high level of ouabain-resistant mutants after exposure to solar UV. A level of 1.1 mutants per 10 6 survivors per kJ m −2 was measured for ICR 2A whereas the yield was 4.2 mutants per 10 6 survivors per kJ m −2 for the solar-UV-sensitive cell line. The DRP 512 cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity and mutation induction were restored. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and mutation induction to DRP 512. This gene has been designated SUVCC2.