Journal of the American Chemical Society, 2015-07, Vol.137 (28), p.8998-9005
2015
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Spectroscopic Investigations of [FeFe] Hydrogenase Maturated with [(57)Fe2(adt)(CN)2(CO)4](2-)
- Ist Teil von
- Journal of the American Chemical Society, 2015-07, Vol.137 (28), p.8998-9005
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The preparation and spectroscopic characterization of a CO-inhibited [FeFe] hydrogenase with a selectively (57)Fe-labeled binuclear subsite is described. The precursor [(57)Fe2(adt)(CN)2(CO)4](2-) was synthesized from the (57)Fe metal, S8, CO, (NEt4)CN, NH4Cl, and CH2O. (Et4N)2[(57)Fe2(adt)(CN)2(CO)4] was then used for the maturation of the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii, to yield the enzyme selectively labeled at the [2Fe]H subcluster. Complementary (57)Fe enrichment of the [4Fe-4S]H cluster was realized by reconstitution with (57)FeCl3 and Na2S. The Hox-CO state of [2(57)Fe]H and [4(57)Fe-4S]H HydA1 was characterized by Mössbauer, HYSCORE, ENDOR, and nuclear resonance vibrational spectroscopy.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1520-5126DOI: 10.1021/jacs.5b03270Titel-ID: cdi_proquest_miscellaneous_1698390900
- Format
- –
- Schlagworte
- Carbon Monoxide - metabolism, Catalytic Domain, Chlamydomonas reinhardtii - chemistry, Chlamydomonas reinhardtii - enzymology, Chlamydomonas reinhardtii - metabolism, Electron Spin Resonance Spectroscopy, Hydrogenase - antagonists & inhibitors, Hydrogenase - chemistry, Hydrogenase - metabolism, Iron Compounds - chemistry, Iron Isotopes - chemistry, Iron-Sulfur Proteins - antagonists & inhibitors, Iron-Sulfur Proteins - chemistry, Iron-Sulfur Proteins - metabolism, Models, Molecular, Spectroscopy, Mossbauer