Molecular medicine reports, 2015-09, Vol.12 (3), p.3607-3614
2015
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- Autor(en) / Beteiligte
- Titel
- Loquat (Eriobotrya japonica) extract prevents dexamethasone-induced muscle atrophy by inhibiting the muscle degradation pathway in Sprague Dawley rats
- Ist Teil von
- Molecular medicine reports, 2015-09, Vol.12 (3), p.3607-3614
- Ort / Verlag
- Greece: D.A. Spandidos
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In the Orient, loquat (Eriobotrya japonica) extract (LE) is widely used in teas, food and folk medicines. The leaves of the loquat tree have been used for generations to treat chronic bronchitis, coughs, phlegm production, high fever and gastroenteric disorders. One of the major active components of loquat leaves is ursolic acid, which was recently investigated in the context of preventing muscle atrophy. The present study investigated the therapeutic potential of LE on dexamethasone-induced muscle atrophy in rats. Daily intraperitoneal injections of dexamethasone caused muscle atrophy and evidence of muscle atrophy prevention by LE was demonstrated using various assays. In particular, dexamethasone-induced grip strength loss was alleviated by LE and the increase in serum creatine kinase activity, a surrogate marker of muscle damage, caused by dexamethasone injection was reduced by LE. Western blot analysis and immunoprecipitation demonstrated that dexamethasone markedly increased the protein expression levels of muscle ring finger 1 (MuRF1), which causes the ubiquitination and degradation of myosin heavy chain (MyHC), and decreased the protein expression levels of MyHC as well as increased the ubiquitinated MyHC to MyHC ratio. However, LE reduced the dexamethasone-induced protein expression levels of MuRF1 and ubiquitinated MyHC. Additional experiments revealed that LE supplementation inhibited the nuclear translocation of FoxO1 induced by dexamethasone. These findings suggested that LE prevented dexamethasone-induced muscle atrophy by regulating the FoxO1 transcription factor and subsequently the expression of MuRF1.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1791-2997eISSN: 1791-3004DOI: 10.3892/mmr.2015.3821Titel-ID: cdi_proquest_miscellaneous_1697210652
- Format
- –
- Schlagworte
- Acids, Aging, Animals, Atrophy, Atrophy, Muscular, Bronchitis, Causes of, Cell cycle, Complications and side effects, Creatine, Creatine kinase, Dexamethasone, Dietary supplements, Eriobotrya - chemistry, Fever, Folk medicine, Food, forkhead box O1, Forkhead Transcription Factors - metabolism, FOXO1 protein, Health aspects, Immunoprecipitation, Insulin, Kinases, Leaves, loquat extract, Medicinal plants, muscle atrophy, muscle degradation, Muscle Proteins - metabolism, muscle ring finger 1, Muscles - drug effects, Muscles - metabolism, Muscles - pathology, Muscular Atrophy - chemically induced, Muscular Atrophy - metabolism, Muscular Atrophy - pathology, Muscular Atrophy - prevention & control, Musculoskeletal system, Myosin, Nerve Tissue Proteins - metabolism, Nuclear transport, Plant Extracts - chemistry, Plant Extracts - therapeutic use, Prevention, Protein expression, Protein synthesis, Proteins, Proteolysis - drug effects, Rats, Sprague-Dawley, Rodents, Rosaceae, Steroids, Studies, Transcription factors, Tripartite Motif Proteins, Ubiquitin - metabolism, Ubiquitin-Protein Ligases - metabolism, Ubiquitination, Ursolic acid