Details

Autor(en) / Beteiligte

• Chu, Haiyan
• Xia, Liping
• Qiu, Xiaonan
• Gu, Dongying
• Zhu, Linjun
• Jin, Jing
• Hui, Gaoyun
• Hua, Qiuhan
• Du, Mulong
• Tong, Na
• Chen, Jinfei
• Zhang, Zhengdong
• Wang, Meilin

Titel

Genetic variants in noncoding PIWI‐interacting RNA and colorectal cancer risk

Ist Teil von

• Cancer, 2015-06, Vol.121 (12), p.2044-2052

Ort / Verlag

United States

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• BACKGROUND PIWI‐interacting RNAs (piRNAs), which are a novel type of identified small noncoding RNA (ncRNA), play a crucial role in germline development and carcinogenesis. METHODS By systematically screening all known piRNAs, the authors identified 7 common single nucleotide polymorphisms (SNPs) in 9 piRNAs. Associations between these selected SNPs and the risk of colorectal cancer (CRC) were detected in a case‐control study. A quantitative real‐time polymerase chain reaction assay was used to evaluate messenger RNA (mRNA) expression levels of piR‐015551 and of the long ncRNA (lncRNA) LNC00964‐3 in 88 pairs of tissue samples. RESULTS The assay revealed that reference SNP rs11776042 in piR‐015551 was significantly associated with a decreased risk of CRC in an additive model (P = .020). However, this protective effect was not significant after correction for multiple comparisons (test for the false discovery rate; P = .140). Furthermore, the authors observed that mRNA expression levels of LNC00964‐3 (an lncRNA that included the piR‐015551 sequence but not piR‐015551) were significantly lower in CRC tissues than in corresponding normal tissues (P = 1.5 × 10−5 for LNC00964‐3; P = .899 for piR‐015551). Correlation analysis revealed that piR‐015551 expression was positively correlated with expression levels of LNC00964‐3 (CRC tissues: r = 0.574, P = 5.13 × 10−9; normal tissues: r = 0.601, P = 5.76 × 10−10). Moreover, rs11776042 was not significantly correlated with mRNA expression levels of piR‐015551 or LNC00964‐3 (all P > .05). CONCLUSIONS The current findings reveal the possibility that piR‐015551 may be generated from LNC00964‐3, which may be involved in the development of CRC. Cancer 2015;121:2044–2052. © 2015 American Cancer Society. The PIWI‐interacting RNA 015551 reference single nucleotide polymorphism rs11776042, which is located in the long noncoding RNA LNC00964‐3, may decrease the risk of colorectal cancer, and the expression level of this interacting RNA has a positive correlation with that of LNC00964‐3.