Details

Autor(en) / Beteiligte

• Sun, Zhengwang
• Park, Sang-Yong
• Hwang, Eunson
• Zhang, Mengyang
• Jin, Fengxie
• Zhang, Baochun
• Yi, Tae Hoo

Titel

Salvianolic Acid B Protects Normal Human Dermal Fibroblasts Against Ultraviolet B Irradiation-Induced Photoaging Through Mitogen-Activated Protein Kinase and Activator Protein-1 Pathways

Ist Teil von

• Photochemistry and photobiology, 2015-07, Vol.91 (4), p.879-886

Ort / Verlag

United States: Blackwell Publishing Ltd

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Exposure to ultraviolet (UV) light causes increased matrix metalloproteinase (MMP) activity and decreased collagen synthesis, leading to skin photoaging. Salvianolic acid B (SAB), a polyphenol, was extracted and purified from salvia miltiorrhiza. We assessed effects of SAB on UVB‐induced photoaging and investigated its molecular mechanism of action in UVB‐irradiated normal human dermal fibroblasts. Our results show that SAB significantly inhibited the UVB‐induced expression of metalloproteinases‐1 (MMP‐1) and interleukin‐6 (IL‐6) while promoting the production of type I procollagen and transforming growth factor β1 (TGF‐β1). Moreover, treatment with SAB in the range of 1–100 μg/mL significantly inhibited UVB‐induced extracellular signal‐regulated kinase (ERK), Jun N‐terminal kinase (JNK) and p38 phosphorylation, which resulted in decreasing UVB‐induced phosphorylation of c‐Fos and c‐Jun. These results indicate that SAB downregulates UV‐induced MMP‐1 expression by inhibiting Mitogen‐activated protein kinase (MAPK) signaling pathways and activator protein‐1 (AP‐1) activation. Our results suggest a potential use for SAB in skin photoprotection. UV causes increased matrix metalloproteinase (MMP) activity and decreased collagen synthesis, leading to skin photoaging. We found that salvianolic acid B (SAB) significantly inhibited the UVB‐induced expression of metalloproteinases‐1 (MMP‐1) while promoting the production of transforming growth factor β1 (TGF‐β1). Moreover, SAB strongly inhibited UVB‐induced extracellular signal‐regulated kinase (ERK), Jun N‐terminal kinase (JNK) and p38 phosphorylation, which resulted in decreasing UVB‐induced phosphorylation of c‐Fos and c‐Jun. These results indicate that SAB down‐regulates UV‐induced MMP‐1 expression by inhibiting mitogen‐activated protein kinase (MAPK) signaling pathways and activator protein‐1 (AP‐1) activation.