Details

Autor(en) / Beteiligte

• Hsu, Chao-Kai
• Huang, Hsin-Yu
• Chen, Wan-Rung
• Nishie, Wataru
• Ujiie, Hideyuki
• Natsuga, Ken
• Fan, Shu-Ting
• Wang, Hsi-Kai
• Lee, Julia Yu-Yun
• Tsai, Wei-Lun
• Shimizu, Hiroshi
• Cheng, Chao-Min

Titel

Paper-Based ELISA for the Detection of Autoimmune Antibodies in Body FluidThe Case of Bullous Pemphigoid

Ist Teil von

• Analytical chemistry (Washington), 2014-05, Vol.86 (9), p.4605-4610

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Bullous pemphigoid (BP), a common autoimmune blistering disease, is increasing in incidence and conveys a high mortality. Detection of autoantibodies targeting the noncollagenous 16A (NC16A) domain of type XVII collagen using enzyme-linked immunosorbent assay (ELISA) has demonstrated high sensitivity and specificity for diagnosing BP. We have developed a rapid, low-cost, and widely applicable ELISA-based system to detect the NC16A autoimmune antibody and then diagnose and monitor BP disease activity using a piece of filter paper, a wax-printer, and NC16A antigens. Both sera and/or blister fluids from 14 untreated BP patients were analyzed. The control group included healthy volunteers and patients with other blistering disorders such as pemphigus vulgaris. In our established paper-based ELISA (P-ELISA) system, only 2 μL of serum or blister fluid and 70 min were required to detect anti-NC16A autoimmune antibodies. The relative color intensity was significantly higher in the BP group than in the control groups when using either serum (P < 0.05) or blister fluid (P < 0.001) specimens from BP patients. The results of P- ELISA were moderately correlated with the titer of the commercial ELISA kit (MBL, Japan) (rho = 0.5680, P = 0.0011). This newly developed system allows for rapid and convenient diagnosis and/or monitoring of BP disease activity.