Journal of clinical pathology, 2012-05, Vol.65 (5), p.394-398
2012
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- Autor(en) / Beteiligte
- Titel
- Infantile haemangioma expresses embryonic stem cell markers
- Ist Teil von
- Journal of clinical pathology, 2012-05, Vol.65 (5), p.394-398
- Ort / Verlag
- London: BMJ Publishing Group Ltd and Association of Clinical Pathologists
- Erscheinungsjahr
- 2012
- Quelle
- BMJ Journals Archiv - DFG Nationallizenzen
- Beschreibungen/Notizen
- BackgroundThe origin of infantile haemangioma (IH) remains enigmatic. A primitive mesodermal phenotype origin of IH with the ability to differentiate down erythropoietic and terminal mesenchymal lineages has recently been demonstrated.AimsTo investigate the expression of human embryonic stem cell (hESC) markers in IH and to determine whether IH-derived cells have the functional capacity to form teratoma in vivo.MethodsImmunohistochemical staining and quantitative reverse transcription PCR were used to investigate the expression of hESC markers in IH biopsies. The ability of cells derived from proliferating IH to form teratomas in a mouse xenograft model was investigated.ResultsThe hESC markers, Oct-4, STAT-3 and stage-specific embryonic antigen 4 were collectively expressed on the endothelium of proliferating IH lesions, whereas Nanog was not. Nanog was expressed by cells in the interstitium and these cells did not express Oct-4, stage-specific embryonic antigen 4 or STAT-3. Proliferating IH-derived cells were unable to form teratomas in severely compromised immunodeficient/non-obese diabetic mice.ConclusionThe novel expression of hESC on two different populations of cells in proliferating IH and their inability to form teratomas in vivo infer the presence of a primitive cellular origin for IH downstream from hESC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9746eISSN: 1472-4146DOI: 10.1136/jclinpath-2011-200462Titel-ID: cdi_proquest_miscellaneous_1687663949
- Format
- –
- Schlagworte
- Angiogenesis, Animals, Biological and medical sciences, biological sciences, Biomarkers, Tumor - genetics, Biopsy, cancer research, cancer stem cells, cell biology, Cell Proliferation, Child, embryonic stem cells, Embryonic Stem Cells - metabolism, Endothelium, Gene Expression, Genes, Genotype & phenotype, haemogenic endothelium, head and neck cancer, Hemangioma - genetics, Hemangioma - metabolism, Hemangioma - pathology, Humans, Infant, infantile haemangioma, Investigative techniques, diagnostic techniques (general aspects), Medical sciences, Mice, Mice, Inbred NOD, Mice, Nude, Mice, SCID, Neoplasm Transplantation, Octamer Transcription Factor-3 - genetics, Octamer Transcription Factor-3 - metabolism, Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques, Population, primitive mesoderm, Reverse Transcriptase Polymerase Chain Reaction, Skin Neoplasms - genetics, Skin Neoplasms - metabolism, Skin Neoplasms - pathology, Stage-Specific Embryonic Antigens - genetics, Stage-Specific Embryonic Antigens - metabolism, STAT3 Transcription Factor - genetics, STAT3 Transcription Factor - metabolism, Stem cells, Teratoma - etiology, Teratoma - pathology, Transcription factors, Tumors, tumour biology, tumour markers, Xenograft Model Antitumor Assays