Nutrition research (New York, N.Y.), 2013-09, Vol.33 (9), p.733-742
2013
Details
- Autor(en) / Beteiligte
- Titel
- Genetic ablation of carotene oxygenases and consumption of lycopene or tomato powder diets modulate carotenoid and lipid metabolism in mice
- Ist Teil von
- Nutrition research (New York, N.Y.), 2013-09, Vol.33 (9), p.733-742
- Ort / Verlag
- New York, NY: Elsevier Inc
- Erscheinungsjahr
- 2013
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Carotene-15,15′-monooxygenase (CMO-I) cleaves β -carotene to form vitamin A, whereas carotene-9′,10′-monooxygenase (CMO-II) preferentially cleaves non–provitamin A carotenoids. Recent reports indicate that β -carotene metabolites regulate dietary lipid uptake, whereas lycopene regulates peroxisome proliferator-activated receptor expression. To determine the physiologic consequences of carotenoids and their interactions with CMO-I and CMO-II, we characterized mammalian carotenoid metabolism, metabolic perturbations, and lipid metabolism in female CMO-I−/− and CMO-II−/− mice fed lycopene or tomato-containing diets for 30 days. We hypothesized that there would be significant interactions between diet and genotype on carotenoid accumulation and lipid parameters. CMO-I−/− mice had higher levels of leptin, insulin, and hepatic lipidosis but lower levels of serum cholesterol. CMO-II−/− mice had increased tissue lycopene and phytofluene accumulation, reduced insulin-like growth factor 1 levels and cholesterol levels, but elevated liver lipids and cholesterol compared with wild-type mice. The diets did not modulate these genotypic perturbations, but lycopene and tomato powder significantly decreased serum insulin-like growth factor 1. Tomato powder also increased hepatic peroxisome proliferator-activated receptor expression, independent of genotype. These data point to the pleiotropic actions of CMO-I and CMO-II supporting a strong role of these proteins in regulating tissue carotenoid accumulation and the lipid metabolic phenotype as well as tomato carotenoid-independent regulation of lipid metabolism.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0271-5317eISSN: 1879-0739DOI: 10.1016/j.nutres.2013.07.007Titel-ID: cdi_proquest_miscellaneous_1687661220
- Format
- –
- Schlagworte
- Adipose Tissue - metabolism, Animals, beta Carotene - administration & dosage, beta Carotene - blood, beta-carotene, beta-Carotene 15,15'-Monooxygenase - genetics, beta-Carotene 15,15'-Monooxygenase - metabolism, Biological and medical sciences, Carotenoids - administration & dosage, Carotenoids - blood, Cholesterol, Cholesterol - blood, CMO-I, CMO-II, Diet, dietary fat, Fatty Acid Desaturases - genetics, Fatty Acid Desaturases - metabolism, Feeding. Feeding behavior, Female, Fundamental and applied biological sciences. Psychology, Gastroenterology and Hepatology, Genotype, Hormones - blood, Inflammation - pathology, insulin, insulin-like growth factor I, Insulin-Like Growth Factor I - metabolism, lipid metabolism, Lipid Metabolism - drug effects, Lipids, liver, Liver - drug effects, Liver - metabolism, Lycopene, Lycopersicon esculentum, Lycopersicon esculentum - chemistry, metabolites, Mice, Mice, Inbred C57BL, Mice, Knockout, Organ Size, oxygenases, Phenotype, Phytoene, Powders - chemistry, proteins, RNA, Messenger - genetics, RNA, Messenger - metabolism, Tomato, tomatoes, Vertebrates: anatomy and physiology, studies on body, several organs or systems, vitamin A