Details

Autor(en) / Beteiligte

• Choi, Eun Bi
• Choi, Jihye
• Bae, Seo Ryung
• Kim, Hyun-Ouk
• Jang, Eunji
• Kang, Byunghoon
• Kim, Myeong-Hoon
• Kim, Byeongyoon
• Suh, Jin-Suck
• Lee, Kwangyeol
• Huh, Yong-Min
• Haam, Seungjoo

Titel

Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport

Ist Teil von

• Nano research, 2015-04, Vol.8 (4), p.1169-1179

Ort / Verlag

Beijing: Tsinghua University Press

Erscheinungsjahr

2015

Quelle

SpringerNature Journals

Beschreibungen/Notizen

• Vesicular pH modulates the function of many organelles and plays a pivotal role in cell metabolism processes such as proliferation and apoptosis. Here, we introduce a simple colorimetric redox-polyaniline nanoindicator, which can detect and quantify a broader biogenic pH range with superior sensitivity compared to pre-established trafficking agents employing one-dimensional turn-on of the fluorescence resonance-energy-transfer （FRET） signal. We fabricated polyaniline- based nanoprobes, which exhibited convertible transition states according to the proton levels, as an in situ indicator of vesicular transport pH. Silica-coated Fe304-MnO heterometal nanoparticles were synthesised and utilised as a metal oxidant to polymerise the aniline monomer. Finally, silica-coated polyaniline nanoparticles with adsorbed cyanine dye fluorophores Cy3 and Cy7 （FPSNIcyB and FPSNIcy7） were fabricated as proton-sensitive nanoindicators. Owing to the selective quenching induced by the local pH variations of vesicular transport, FPSNIcy3 and FPSNIcy7 demonstrated excellent intracellular trafficking and provided sensitive optical indication of minute proton levels.