Details

Autor(en) / Beteiligte

• Barouni, Eleftheria
• Petsi, Theano
• Kanellaki, Maria
• Bekatorou, Argyro
• Koutinas, Athanasios

Titel

Tubular cellulose/starch gel composite as food enzyme storehouse

Ist Teil von

• Food chemistry, 2015-12, Vol.188, p.106-110

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •Preparation of porous cellulose/starch gel (TC/SG) composite for enzyme entrapment.•The composite had reduced pore volume and pore size compared to plain TC.•XRD showed decreased crystallinity with increased SG ratio in the composite.•The composite is suitable carrier for rennin entrapment for dairy applications.•Perspectives in research by composite polyenzyme or cell/enzyme biocatalysts. The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications. The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area. The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes. X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite. The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20min and full coagulation at about 200min), creating perspectives for several applications in food enzyme research and technology.