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Surface chemistry gradients on silicone elastomers for high-throughput modulation of cell-adhesive interfaces
Journal of biomedical materials research. Part A, 2015-06, Vol.103 (6), p.2066-2076
Mohan, Greeshma
Gallant, Nathan D.
2015
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Mohan, Greeshma
Gallant, Nathan D.
Titel
Surface chemistry gradients on silicone elastomers for high-throughput modulation of cell-adhesive interfaces
Ist Teil von
Journal of biomedical materials research. Part A, 2015-06, Vol.103 (6), p.2066-2076
Ort / Verlag
United States: Blackwell Publishing Ltd
Erscheinungsjahr
2015
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
Combinatorial and high‐throughput approaches to screening cell responses to material properties accelerate the speed of discovery and facilitate the identification of cell instructive cues or trends that may be missed by discrete sampling. However, these technologies have not yet been widely applied to materials with tissue‐like stiffness. The fabrication of monotonically varying surface chemistry gradients on polydimethylsiloxane, an elastic biomaterial, and the influence of these engineered surfaces on protein adsorption and adherent cell morphology were explored in this study. Crosslinked networks of polydimethylsiloxane were functionalized with a hydrophobic self‐assembled monolayer and then modified by spatiotemporally regulated ultraviolet ozonolysis to obtain gradients of oxygenated species ranging from ∼10° to ∼100° in water contact angle. Automated microscopy and image analysis of fibroblast cell morphology revealed a strong correlation between cell spreading and hydrophobicity. However, structural and functional analysis of the fibronectin interface indicated a proportional increase in cell spreading with adsorption, but a biphasic relationship with fibronectin conformation, underscoring the complexity of the adhesive interface. This work demonstrates the development of an elastomer surface modification platform that can be extended to future combinatorial studies of biological responses to chemical and mechanical material properties. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2066–2076, 2015.
Sprache
Englisch
Identifikatoren
ISSN: 1549-3296
eISSN: 1552-4965
DOI: 10.1002/jbm.a.35349
Titel-ID: cdi_proquest_miscellaneous_1685832396
Format
–
Schlagworte
Adsorption
,
Animals
,
Biocompatible Materials - pharmacology
,
Biomedical materials
,
Cell Adhesion - drug effects
,
Cell Movement - drug effects
,
Combinatorial analysis
,
combinatorial methods
,
Dimethylpolysiloxanes - pharmacology
,
Elastomers
,
Fibronectin
,
Fibronectins - chemistry
,
Fibronectins - pharmacology
,
Humans
,
Hydrophobic and Hydrophilic Interactions
,
hydrophobicity
,
mechanotransduction
,
Mice
,
Morphology
,
NIH 3T3 Cells
,
PDMS
,
Protein Conformation
,
Silicone Elastomers - pharmacology
,
Spreading
,
Surface chemistry
,
Surgical implants
,
Wettability
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