Details

Autor(en) / Beteiligte

• Teo, Ailing
• Mantalaris, Athanasios
• Song, Kedong
• Lim, Mayasari

Titel

novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells

Ist Teil von

• Biotechnology letters, 2014-05, Vol.36 (5), p.947-960

Ort / Verlag

Dordrecht: Springer-Verlag

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 10⁵ cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.