Details

Autor(en) / Beteiligte

• Motemani, Yahya
• Greulich, Christina
• Khare, Chinmay
• Lopian, Michael
• Buenconsejo, Pio John S.
• Schildhauer, Thomas A.
• Ludwig, Alfred
• Köller, Manfred

Titel

Adherence of human mesenchymal stem cells on Ti and TiO2 nano-columnar surfaces fabricated by glancing angle sputter deposition

Ist Teil von

• Applied surface science, 2014-02, Vol.292, p.626-631

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2014

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Adherence and proliferation of human mesenchymal stem cells (hMSCs) on Ti and TiO2 nano-columnar surfaces fabricated by glancing angle deposition.•Observation of very small pseudopodia with width of approximately 20–35nm and length varying from 20 to 200nm between the nano-columns.•Promising nano-columnar surfaces for bio-functionalization or drug loading of implants.•Promising nano-columnar morphologies for antibacterial properties. The interaction of human mesenchymal stem cells (hMSCs) with Ti and TiO2 nano-columnar surfaces fabricated using glancing angle sputter deposition was investigated. The adherence and proliferation of hMSCs on different nano-columnar surfaces, including vertical columns, slanted columns and chevrons, were examined with calcein-acetoxymethyl ester fluorescence staining and scanning electron microscopy. For comparison, adherence of hMSCs on compact, dense films was also studied. After 24h and 7 days, adherent and viable cells were observed on both, Ti nano-columns as well as dense Ti films, which confirms the biocompatibility of these nanostructures. Very small pseudopodia with width of approximately 20–35nm and length varying from 20 to 200nm were observed between the nano-columns, independent of the type of the nano-columnar morphology. Large inter-column spacing and effectively increased surface area make these nanostructures promising candidates for bio-functionalization or drug loading on the surface of Ti-based implants.