Details

Autor(en) / Beteiligte

• Zhang, Li-Li
• Guo, Yan-Jun
• Zhao, Chun-Na
• Gao, Jian-Yun

Titel

Effects and mechanism of miR-214 on hepatocellular carcinoma

Ist Teil von

• Asian Pacific journal of tropical medicine, 2015-05, Vol.8 (5), p.392-398

Ort / Verlag

India: Elsevier B.V

Erscheinungsjahr

2015

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Objective:To explore the role of miR-214 in the progression of hepatocellular carcinoma（HCC） and its inhibitory mechanisms in depressing the signaling pathway of j3-catenin.this study was conducted.Methods:We ectopically expressed miR-214 in HepG2 cells to obtain cell lines Lv-miR-214-HepG2 and their control Lv-control-HepG2.Differences between the two cell lines were compared in cell growth,proliferation,colony forming ability and cell cycles.RT-PCR method was applied for the quantification of β-catenin mRNA expression.Western-blot method was applied for the determination of the protein level of β-catenin and their downstream targets（ie.Cyclin D1,c-Myc and TCF-1）.The effect of miR-214 on cells was further explored through RNA interference and restoring miR-214 expression.Results:In comparison with negative（Lv-control-HepG2） and blank（HepG2） control,a significant inhibition of cell growth and proliferation caused by miR-214 was observed after 4872h of cell culture experiments（P<0.05）.The miR-214 treatment resulted in a colony forming efficiency of（23.28±3.26）%,which was significantly lower than that of negative control[（51.31±3.97）%]（P<0.05）.According to FCM results,the experimental group,compared with control,showed a higher proportion of cells in G0/G1 phase[（70.32±3.12）%]but a lower proportion in S phase[（18.42±2.90）%]（P<0.05）.The MTT assay demonstrated a significant inhibition of the proliferation and β-catenin expression of HCC cells compared with control（P<0.05）.while no significant difference was observed after HCC cells being transfected withβ-catenin overexpression plasmid（P>0.05）.By comparing to the RT-PCR and Western-blot results of control,the miR-214 treatment led to a slightly decrease in the β-catenin mRNA expression（P>0.05）.but an extremely inhibition in the protein level of β-catenin and its downstream targets Cyclin Dl,c-Myc.and TCF-1（P<0.05）.Conclusions:miR-214 functions as a suppressor during the progression of HCC,and its inhibitory role was achieved by downregulating β-catenin signaling pathway.