Details

Autor(en) / Beteiligte

• Lawrence, Michael B.
• Berg, Ellen L.
• Butcher, Eugene C.
• Springer, Timothy A.

Titel

Rolling of lymphocytes and neutrophils on peripheral node addressin and subsequent arrest on ICAM‐1 in shear flow

Ist Teil von

• European journal of immunology, 1995-04, Vol.25 (4), p.1025-1031

Ort / Verlag

Weinheim: WILEY‐VCH Verlag GmbH

Erscheinungsjahr

1995

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• We studied leukocyte interactions in shear flow with peripheral lymph node addressin (PNAd), a mixture of glycoproteins expressed on high endothelial venules (HEV) that is required for lymphocyte homing and has been shown to contain a ligand for L‐selectin. T lymphocytes and neutrophils tether and roll on plastic‐immobilized PNAd and E‐selectin at 1.8 dyn/cm2 wall shear stress, but fail to interact with immobilized ICAM‐1, a ligand for LFA‐1 and Mac‐1, at the same flow rate. Cells roll faster on PNAd than on P‐selectin or E‐selectin. L‐selectin mAb inhibit T lymphocyte and neutrophil tethering to PNAd, but do not inhibit T lymphocyte tethering to purified E‐selectin. If allowed to interact with ICAM‐1 under static conditions, phorbol ester‐treated T lymphocytes, but not resting T lymphocytes, are able to form stationary adhesions that withstand the detachment force generated by 36 dyn/cm2 wall shear stress. In contrast, a wall shear stress of 7.3 dyn/cm2 detaches 50% of resting T lymphocytes bound to PNAd. Incubating T lymphocytes on PNAd and ICAM‐1 does not result in adhesion strengthening, suggesting that adhesion through PNAd by L‐selectin does not stimulate lymphocyte LFA‐1 avidity for ICAM‐1. Chemoattractant stimulation of neutrophils or phorbol ester stimulation of lymphoblasts rolling on co‐immobilized PNAd and ICAM‐1 results in rapid arrest and firm sticking, extending the model of sequential selectin‐mediated rolling and subsequent integrin‐mediated firm arrest to lymphocytes and ligands expressed on HEV.