Details

Autor(en) / Beteiligte

• Dong, Cai-Xia
• Zhang, Li-Jiao
• Xu, Rong
• Zhang, Gong
• Zhou, Ying-Biao
• Han, Xiao-Qiang
• Zhang, Ying
• Sun, Yuan-Xia

Titel

Structural characterization and immunostimulating activity of a levan-type fructan from Curcuma kwangsiensis

Ist Teil von

• International journal of biological macromolecules, 2015-06, Vol.77, p.99-104

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• A fructan designated as CKNP with apparent molecular weight of 5.3kD was isolated from the hot water extract of Curcuma kwangsiensis through a combination of ion-exchange chromatography on DEAE 650M and gel filtration on Superdex G-200. CKNP was characterized by chemical derivatization as well as HPLC, GC, and GC–MS technologies. Structural studies revealed that CKNP is composed predominately of fructose (96.8%) and a small amount of glucose (3.2%) with a degree of polymerization (DP) of 30–31. It was deduced to be a levan-type fructan containing a backbone composed of (2→6)-linked β-d-Fruf residues and single β-d-Fruf residues as side chains branched at the O-1 position along the backbone. Preliminary in vitro bioactive tests on RAW 264.7 murine macrophage cells revealed that the levan-type fructan from C. kwangsiensis shows significant immunostimulating activity based on its ability to stimulate macrophage proliferation and enhance phagocytosis.