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MicroRNA-139-5p Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch and IRS1/PI3K/Akt Insulin Signaling Pathways
Journal of cellular biochemistry, 2015-07, Vol.116 (7), p.1195-1204
Mi, Lin
Chen, Yaosheng
Zheng, Xueli
Li, Youlei
Zhang, Qiangling
Mo, Delin
Yang, Gongshe
2015
Details
Autor(en) / Beteiligte
Mi, Lin
Chen, Yaosheng
Zheng, Xueli
Li, Youlei
Zhang, Qiangling
Mo, Delin
Yang, Gongshe
Titel
MicroRNA-139-5p Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch and IRS1/PI3K/Akt Insulin Signaling Pathways
Ist Teil von
Journal of cellular biochemistry, 2015-07, Vol.116 (7), p.1195-1204
Ort / Verlag
United States: Blackwell Publishing Ltd
Erscheinungsjahr
2015
Link zum Volltext
Quelle
Wiley-Blackwell Full Collection
Beschreibungen/Notizen
ABSTRACT MicroRNAs (miRNAs) participate in the regulation of adipogenesis. Identification of the full repertoire of miRNAs expressed in adipose tissue is likely to significantly improve our understanding of adipose tissue growth and development. Here, miR‐139–5p was identified as an inhibitor of 3T3‐L1 adipocyte differentiation with significantly down‐regulating the expression levels of adipogenic marker genes PPAR γ (P < 0.01), aP2 (P < 0.01) and FAS (P < 0.01). Importantly, flow cytometry and EdU incorporation assay indicated that this inhibition was partly due to the dysfunction of clonal expansion. Furthermore, we firstly demonstrated that miR‐139–5p blocked adipogenesis via directly targeted the 3' untranslated regions (UTRs) of Notch1 and IRS1 mRNAs, a key member of Notch signaling and IRS1/PI3K/Akt insulin signaling, respectively. In addition, the overexpression of Notch1 or IRS1 partially restored the suppressive effects miR‐139–5p on differentiation of 3T3‐L1 cells. To our knowledge, this was the first report that miR‐139–5p functioned negatively by targeting Notch1 and IRS1 during 3T3‐L1 adipogenesis, regulating the transition from clonal expansion to terminal differentiation. J. Cell. Biochem. 116: 1195–1204, 2015. © 2014 Wiley Periodicals, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0730-2312
eISSN: 1097-4644
DOI: 10.1002/jcb.25065
Titel-ID: cdi_proquest_miscellaneous_1680959216
Format
–
Schlagworte
3T3 Cells
,
3T3-L1
,
ADIPOCYTE DIFFERENTIATION
,
Adipogenesis
,
Animals
,
Down-Regulation
,
Insulin Receptor Substrate Proteins - genetics
,
IRS1/PI3K/Akt INSULIN SIGNALING
,
Mice
,
MicroRNAs - metabolism
,
miR-139-5p
,
NOTCH SIGNALING
,
PPAR gamma - metabolism
,
Receptors, Notch - genetics
,
Signal Transduction
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