Details

Autor(en) / Beteiligte

• Menzfeld, Christiane
• John, Michael
• van Rossum, Denise
• Regen, Tommy
• Scheffel, Jörg
• Janova, Hana
• Götz, Alexander
• Ribes, Sandra
• Nau, Roland
• Borisch, Angela
• Boutin, Philippe
• Neumann, Konstantin
• Bremes, Vanessa
• Wienands, Jürgen
• Reichardt, Holger M.
• Lühder, Fred
• Tischner, Denise
• Waetzig, Vicky
• Herdegen, Thomas
• Teismann, Peter
• Greig, Iain
• Müller, Michael
• Pukrop, Tobias
• Mildner, Alexander
• Kettenmann, Helmut
• Brück, Wolfgang
• Prinz, Marco
• Rotshenker, Shlomo
• Weber, Martin S.
• Hanisch, Uwe-Karsten

Titel

Tyrphostin AG126 exerts neuroprotection in CNS inflammation by a dual mechanism

Ist Teil von

• Glia, 2015-06, Vol.63 (6), p.1083-1099

Ort / Verlag

United States: Blackwell Publishing Ltd

Erscheinungsjahr

2015

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The putative protein tyrosine kinase (PTK) inhibitor tyrphostin AG126 has proven beneficial in various models of inflammatory disease. Yet molecular targets and cellular mechanisms remained enigmatic. We demonstrate here that AG126 treatment has beneficial effects in experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis. AG126 alleviates the clinical symptoms, diminishes encephalitogenic Th17 differentiation, reduces inflammatory CNS infiltration as well as microglia activation and attenuates myelin damage. We show that AG126 directly inhibits Bruton's tyrosine kinase (BTK), a PTK associated with B cell receptor and Toll‐like receptor (TLR) signaling. However, BTK inhibition cannot account for the entire activity spectrum. Effects on TLR‐induced proinflammatory cytokine expression in microglia involve AG126 hydrolysis and conversion of its dinitrile side chain to malononitrile (MN). Notably, while liberated MN can subsequently mediate critical AG126 features, full protection in EAE still requires delivery of intact AG126. Its anti‐inflammatory potential and especially interference with TLR signaling thus rely on a dual mechanism encompassing BTK and a novel MN‐sensitive target. Both principles bear great potential for the therapeutic management of disturbed innate and adaptive immune functions. GLIA 2015;63:1083–1099 Main Points Tyrphostin AG126 has beneficial effects in mice with experimental autoimmune encephalomyelitis, by reducing inflammation and tissue damage. AG126 directly inhibits Bruton's tyrosine kinase but also acts on a malononitrile‐sensitive target.