Details

Autor(en) / Beteiligte

• Justino, Priscilla F. C.
• Melo, Luis F. M.
• Nogueira, Andre F.
• Morais, Cecila M.
• Mendes, Walber O.
• Franco, Alvaro X.
• Souza, Emmanuel P.
• Ribeiro, Ronaldo A.
• Souza, Marcellus H. L. P.
• Soares, Pedro Marcos Gomes

Titel

Regulatory role of Lactobacillus acidophilus on inflammation and gastric dysmotility in intestinal mucositis induced by 5-fluorouracil in mice

Ist Teil von

• Cancer chemotherapy and pharmacology, 2015-03, Vol.75 (3), p.559-567

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Purpose Lactobacillus acidophilus is widely used for gastrointestinal disorders, but its role in inflammatory conditions like in chemotherapy-induced mucositis is unclear. Here, we report the effect of L. acidophilus on 5-fluorouracil-induced (5-FU) intestinal mucositis in mice. Methods Mice weighing 25–30 g ( n  = 8) were separated into three groups, saline, 5-FU, and 5-FU +  L. acidophilus (5-FU-La) (16 × 10 9  CFU/kg). In the 5-FU-La group, L. acidophilus was administered concomitantly with 5-FU on the first day and alone for two additional days. Three days after the last administration of L. acidophilus , the animals were euthanized and the jejunum and ileum were removed for histopathological assessment and for evaluation of levels of myeloperoxidase activity, sulfhydryl groups, nitrite, and cytokines (TNF-α, IL-1β, CXCL-1, and IL-10). In addition, we investigated gastric emptying using spectrophotometry after feeding a 1.5-ml test meal by gavage and euthanasia. Data were submitted to ANOVA and Bonferroni’s test, with the level of significance at p  < 0.05. Results Intestinal mucositis induced by 5-FU significantly ( p  < 0.05) reduced the villus height–crypt depth ratio and GSH concentration and increased myeloperoxidase activity and the nitrite concentrations compared with the control group. Furthermore, 5-FU significantly ( p  < 0.05) increased cytokine (TNF-α, IL-1β, and CXCL-1) concentrations and decreased IL-10 concentrations compared with the control group. 5-FU also significantly ( p  < 0.05) delayed gastric emptying and gastrointestinal transit compared with the control group. All of these changes were significantly ( p  < 0.05) reversed by treatment with L. acidophilus. Conclusions Lactobacillus acidophilus improves the inflammatory and functional aspects of intestinal mucositis induced by 5-FU.