Details

Autor(en) / Beteiligte

• Liu, Yujie
• Luo, Rifang
• Shen, Fangyu
• Tang, Linlin
• Wang, Jin
• Huang, Nan

Titel

Construction of mussel-inspired coating via the direct reaction of catechol and polyethyleneimine for efficient heparin immobilization

Ist Teil von

• Applied surface science, 2015-02, Vol.328, p.163-169

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Catechol (CA) and PEI copolymerization was a mimetic and dopamine-like coating method.•CA/PEI film provided amine groups and was effective in heparin immobilization.•CA/PEI coating could inhibit smooth muscle cell proliferation.•CA/PEI coating did not show any significant cytotoxicity to endothelial cell. Dopamine could self-polymerize to form the coating on various substrates and the co-existence of catechols and amines was crucial in performing such polymerization process. In this work, a mimetic approach of coating formation was carried out based on the co-polymerization of catechol (CA) and polyethyleneimine (PEI). Mussel-inspired CA/PEI coating was deposited on 316L stainless steel (SS). Fourier transform infrared spectra (FTIR) and X-ray photoelectron spectroscopy (XPS) demonstrated the successful coating formation. QCM measurement showed good affinity of heparin immobilization on CA/PEI coating surface ascribed to the amine groups. Herein, vascular cell-material interactions like endothelial cells (ECs) and smooth muscle cells (SMCs) were also investigated. Interestingly, CA/PEI and heparin modified coatings presented no cytotoxicity to ECs, however to a certain extent, decreased SMCs proliferation. Moreover, heparin-binding surface presented significant anti-platelet adhesion and activation properties. These results effectively suggested that the mussel-inspired CA/PEI coating might be promising when served as a platform for biomolecule immobilization.