Details

Autor(en) / Beteiligte

• Hauptmann, Nicole
• Pion, Marjorie
• Muñoz-Fernández, María-Ángeles
• Komber, Hartmut
• Werner, Carsten
• Voit, Brigitte
• Appelhans, Dietmar

Titel

Ni(II)-NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical Properties and First In Vitro Study of Polyplexes Formed with HIV-Derived Peptides

Ist Teil von

• Macromolecular bioscience, 2013-05, Vol.13 (5), p.531-538

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)‐nitrilo(triacetic acid)‐modified poly(ethylene imine)‐maltose (Ni‐NTA‐DG) is realized and evaluated as complexation agent against His‐tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5–6.0, and also up to 50 mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte‐derived immature dendritic cells. In summary, Ni‐NTA‐DG represents a promising (delivery) platform for forthcoming in vitro applications. Ni‐NTA‐modified dendritic glycopolymers show high potential as a delivery system for coupling His‐tagged antigens into dendritic cells (DC) under in vitro conditions. Ni‐NTA‐units prove to induce antigen uptake in DC. Stable polyplexes are demonstrated against slightly lower pH (pH ≥ 6.0) and different imidazole concentrations (10–50 µM) depending on the kind of polyplex.