Plant physiology (Bethesda), 2015-05, Vol.168 (1), p.205-221
2015
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- Autor(en) / Beteiligte
- Titel
- The Conserved Endoribonuclease YbeY Is Required for Chloroplast Ribosomal RNA Processing in Arabidopsis
- Ist Teil von
- Plant physiology (Bethesda), 2015-05, Vol.168 (1), p.205-221
- Ort / Verlag
- United States: American Society of Plant Biologists
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Maturation of chloroplast ribosomal RNAs (rRNAs) comprises several endoribonucleolytic and exoribonucleolytic processing steps. However, little is known about the specific enzymes involved and the cleavage steps they catalyze. Here, we report the functional characterization of the single Arabidopsis (Arabidopsis thaliana) gene encoding a putative YbeY endoribonuclease.AtYbeYnull mutants are seedling lethal, indicating thatAtYbeYfunction is essential for plant growth. Knockdown plants display slow growth and show pale-green leaves. Physiological and ultrastructural analyses ofatybeYmutants revealed impaired photosynthesis and defective chloroplast development. Fluorescent microcopy analysis showed that, when fused with the green fluorescence protein, AtYbeY is localized in chloroplasts. Immunoblot and RNA gel-blot assays revealed that the levels of chloroplast-encoded subunits of photosynthetic complexes are reduced inatybeYmutants, but the corresponding transcripts accumulate normally. In addition, atybeY mutants display defective maturation of both the 5ʹ and 3ʹ ends of 16S, 23S, and 4.5S rRNAs as well as decreased accumulation of mature transcripts from the transfer RNA genes contained in the chloroplast rRNA operon. Consequently, mutant plants show a severe deficiency in ribosome biogenesis, which, in turn, results in impaired plastid translational activity. Furthermore, biochemical assays show that recombinant AtYbeY is able to cleave chloroplast rRNAs as well as messenger RNAs and transfer RNAs in vitro. Taken together, our findings indicate that AtYbeY is a chloroplast-localized endoribonuclease that is required for chloroplast rRNA processing and thus for normal growth and development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889eISSN: 1532-2548DOI: 10.1104/pp.114.255000Titel-ID: cdi_proquest_miscellaneous_1677887685
- Format
- –
- Schlagworte
- Amino Acid Sequence, Arabidopsis - enzymology, Arabidopsis - genetics, Arabidopsis Proteins - chemistry, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Base Sequence, Chlorophylls, Chloroplasts, Chloroplasts - metabolism, Chloroplasts - ultrastructure, Conserved Sequence, Endoribonucleases - chemistry, Endoribonucleases - genetics, Endoribonucleases - metabolism, Gels, Gene Expression Regulation, Plant, GENES, DEVELOPMENT, AND EVOLUTION, Genes, Plant, Genome, Plant, Green Fluorescent Proteins - metabolism, Messenger RNA, Molecular Sequence Data, Mutation - genetics, Phenotype, Photosynthesis, Plant Leaves - physiology, Plants, Plastids, Polyribosomes - metabolism, Protein Subunits - metabolism, Protein Transport, Ribosomes, RNA, RNA Processing, Post-Transcriptional - genetics, RNA, Chloroplast - genetics, RNA, Messenger - genetics, RNA, Messenger - metabolism, RNA, Ribosomal - genetics, Sequence Homology, Amino Acid, Subcellular Fractions - metabolism, Thylakoids, Transfer RNA