Details

Autor(en) / Beteiligte

• Bittencourt, L.S.
• Machado, D.C.
• Machado, M.M.
• Dos Santos, G.F.F.
• Algarve, T.D.
• Marinowic, D.R.
• Ribeiro, E.E.
• Soares, F.A.A.
• Barbisan, F.
• Athayde, M.L.
• Cruz, I.B.M.

Titel

The protective effects of guaraná extract (Paullinia cupana) on fibroblast NIH-3T3 cells exposed to sodium nitroprusside

Ist Teil von

• Food and chemical toxicology, 2013-03, Vol.53, p.119-125

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] ► Nitric oxide generated by sodium nitroprusside causes oxidative stress. ► Guaraná extracts decreased in vitro sodium nitroprusside cytotoxic effect. ► Guaraná extracts decreased in vitro oxidative stress of cells exposed to sodium nitroprusside. The antioxidant effects of the hydro-alcoholic guaraná extract (Paullinia cupana var. sorbilis Mart.) on nitric oxide (NO) and other compounds generated from the degradation of sodium nitroprusside (SNP) in an embryonic fibroblast culture (NIH-3T3 cells) were evaluated. The guaraná bioactive compounds were initially determined by high-performance liquid chromatography: caffeine=12.240mg/g, theobromine=6.733mg/g and total catechins=4.336mg/g. Cells were exposed to 10μM SNP during a 6h period because the cells exhibited >90% mortality at this concentration. Guaraná was added to the cultures in five concentrations (0.5, 1, 5, 10 and 20mg/mL). The guaraná antioxidant effect was evaluated by viability assays, biochemical oxidation [lipid peroxidation, catalase and superoxide dismutase (SOD) activity] and genotoxicity (DNA Comet assay) analysis. Additionally, oxidative stress was evaluated by a 2,7-dihydrodichlorofluorescein diacetate fluorescence assay. Guaraná reverted the SNP toxicity mainly at lower concentrations (<5mg), which decreased cell mortality, lipid peroxidation, DNA damage and cell oxidative stress as well as increased the SOD levels. These results demonstrate that guaraná has an antioxidant effect on NO metabolism in situations with higher cellular NO levels.