Molecular medicine reports, 2015-07, Vol.12 (1), p.1342-1346
2015
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- Autor(en) / Beteiligte
- Titel
- Carbamylated low-density lipoprotein attenuates glucose uptake via a nitric oxide-mediated pathway in rat L6 skeletal muscle cells
- Ist Teil von
- Molecular medicine reports, 2015-07, Vol.12 (1), p.1342-1346
- Ort / Verlag
- Greece: D.A. Spandidos
- Erscheinungsjahr
- 2015
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- Carbamylation is a cyanate-mediated posttranslational modification. We previously reported that carbamylated low-density lipoprotein (cLDL) increases reactive oxygen species and apoptosis via a lectin-like oxidized LDL receptor mediated pathway in human umbilical vein endothelial cells. A recent study reported an association between cLDL and type 2 diabetes mellitus (T2DM). In the current study, the effects of cLDL on glucose transport were explored in skeletal muscle cells. The effect of cLDL on glucose uptake, glucose transporter 4 (GLUT4) translocation, and signaling pathway were examined in cultured rat L6 muscle cells using 2-deoxyglucose uptake, immunofluorescence staining and western blot analysis. The quantity of nitric oxide (NO) was evaluated by the Griess reaction. The effect of native LDL (nLDL) from patients with chronic renal failure (CRF-nLDL) on glucose uptake was also determined. It was observed that cLDL significantly attenuated glucose uptake and GLUT4 translocation to the membrane, which was mediated via the increase in inducible nitric oxide synthase (iNOS)-induced NO production. Tyrosine nitration of the insulin receptor substrate-1 (IRS-1) was increased. It was demonstrated that CRF-nLDL markedly reduced glucose uptake compared with nLDL from healthy subjects. Collectively, these findings indicate that cLDL, alone, attenuates glucose uptake via NO-mediated tyrosine nitration of IRS-1 in L6 rat muscle cells and suggests the possibility that cLDL is involved in the pathogenesis of T2DM.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1791-2997eISSN: 1791-3004DOI: 10.3892/mmr.2015.3481Titel-ID: cdi_proquest_miscellaneous_1673792978
- Format
- –
- Schlagworte
- Animals, Apoptosis, Apoptosis - genetics, carbamylated low-density lipoprotein, Carbamylation, Deoxyglucose, Diabetes, Diabetes mellitus, Diabetes Mellitus, Type 2 - metabolism, Diabetes Mellitus, Type 2 - pathology, Endothelial cells, Genetic aspects, Glucose, Glucose - metabolism, Glucose transport, Glucose transporter, Glucose Transporter Type 4 - biosynthesis, Glucose Transporter Type 4 - metabolism, glucose uptake, Humans, Immunofluorescence, inducible nitric oxide synthase, Insulin, Insulin receptor substrate 1, Insulin Receptor Substrate Proteins - metabolism, Insulin resistance, Kidney Failure, Chronic - metabolism, Kidney Failure, Chronic - pathology, Kidneys, Lipoproteins, Lipoproteins, LDL - administration & dosage, Lipoproteins, LDL - metabolism, Low density lipoprotein, Low density lipoproteins, Metabolic Networks and Pathways, Microscopy, Muscle, Skeletal - metabolism, Muscle, Skeletal - pathology, Muscles, Musculoskeletal system, Nitration, Nitric oxide, Nitric Oxide - metabolism, Nitric Oxide Synthase Type II - genetics, Nitric Oxide Synthase Type II - metabolism, Nitric-oxide synthase, Physiological aspects, Proteins, Rats, Reactive oxygen species, Reactive Oxygen Species - metabolism, Renal failure, Rodents, Signal transduction, Skeletal muscle, skeletal muscle cells, Tyrosine, Umbilical vein