Future microbiology, 2015-04, Vol.10 (4), p.489-501
2015
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- Autor(en) / Beteiligte
- Titel
- Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis
- Ist Teil von
- Future microbiology, 2015-04, Vol.10 (4), p.489-501
- Ort / Verlag
- England: Future Medicine Ltd
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (CDT) mediated pathogenic effects in hosts. The molecular mechanisms underlying cholesterol sequestering conferred resistance to CDT-induced DNA double-strand breaks (DSBs) and cell cycle arrest were investigated. Histopathological analysis was conducted for evaluating CDT-induced intestinal inflammation in mouse. CDT actions were attenuated by treatment of cells with methyl-β-cyclodextrin (MβCD). Severe intestinal inflammation induced by CDT treatment was observed in high-cholesterol diet-fed mice, but not in normal diet-fed mice, indicating that cholesterol is essential for CDT intoxication. Our findings demonstrate a molecular link between Campylobacter jejuni CDT and cholesterol, which is crucial to facilitate CDT-induced pathogenesis in hosts.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1746-0913eISSN: 1746-0921DOI: 10.2217/fmb.14.119Titel-ID: cdi_proquest_miscellaneous_1673078536
- Format
- –
- Schlagworte
- Animals, Apoptosis, Bacteria, Bacterial infections, Bacterial toxins, Bacterial Toxins - toxicity, Campylobacter, Campylobacter Infections - pathology, Campylobacter jejuni - metabolism, Cell cycle, Cholesterol, Cholesterol - metabolism, Diet - methods, Disease Models, Animal, Experiments, Flow cytometry, Gram-negative bacteria, Health aspects, Histocytochemistry, Histograms, Host-Pathogen Interactions, Infections, Intestines - pathology, Lipids, Mice, Pathogenesis, Protein expression, Rodents, Standard deviation, Statins, Studies