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Highly sensitive chemiluminescence immunoassay on chitosan membrane modified paper platform using TiO sub(2) nanoparticles/multiwalled carbon nanotubes as label
A highly sensitive chemiluminescence (CL) immunoassay was incorporated into a low-cost microfluidic paper-based analytical device ( mu -PAD) to fabricate a facile paper-based CL immunodevice (denoted as mu -PCLI). This mu -PCLI was constructed by covalently immobilizing capture antibody on a chitosan membrane modified mu -PADs, which was developed by simple wax printing methodology. TiO sub(2) nanoparticles coated multiwalled carbon nanotubes (TiO sub(2)/MWCNTs) were synthesized as an amplification catalyst tag to label signal antibody (Ab sub(2)). After sandwich-type immunoreactions, the TiO sub(2)/MWCNTs were captured on the surface of mu -PADs to catalyze the luminol-p-iodophenol-H sub(2)O sub(2) CL system, which produced an enhanced CL emission. Using prostate-specific antigen as a model analyte, the approach provided a good linear response range from 0.001 to 20 ng/mL with a low detection limit of 0.8 pg/mL under optimal conditions. This mu -PCLI showed good reproducibility, selectivity and stability. The assay results of prostate-specific antigen in clinical serum samples were in good agreement with that obtained by commercially used electrochemiluminescence methods at the Cancer Research Center of Shandong Tumor Hospital (Jinan, Shandong Province, China). This mu -PCLI could be very useful to realize highly sensitive, qualitative point-of-care testing in developing or developed countries. Copyright [copy 2013 John Wiley & Sons, Ltd.