Details

Autor(en) / Beteiligte

• Davydova, V.N.
• Volod’ko, A.V.
• Sokolova, E.V.
• Chusovitin, E.A.
• Balagan, S.A.
• Gorbach, V.I.
• Galkin, N.G.
• Yermak, I.M.
• Solov’eva, T.F.

Titel

The supramolecular structure of LPS–chitosan complexes of varied composition in relation to their biological activity

Ist Teil von

• Carbohydrate polymers, 2015-06, Vol.123, p.115-121

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •Complexes between LPS from Escherihia coli (E-LPS) and Yersinia pseudotuberculosis (Y-LPS) and chitosan (Ch) were prepared.•E-LPS–Ch complexes had different supramolecular structure.•Complexes of Y-LPS–Ch had similar positive ζ-potential regardless of Ch content.•Activity of E-LPS in complex with Ch decreased significantly in contrast to Y-LPS. The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy. The alteration of ζ-potential of E-LPS–Ch complexes from negative to positive values depending on Ch content was detected. The Y-LPS–Ch complexes had similar positive ζ-potentials regardless of Ch content. The transformation of the supramolecular structure of E-LPS after binding with to Ch was revealed. Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected. The ability of LPS–Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied. A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS–Ch complexes.