Details

Autor(en) / Beteiligte

• Zuo, Ruiting
• Zhou, Si
• Zuo, Yuegang
• Deng, Yiwei

Titel

Determination of creatinine, uric and ascorbic acid in bovine milk and orange juice by hydrophilic interaction HPLC

Ist Teil von

• Food chemistry, 2015-09, Vol.182, p.242-245

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •HILIC was developed for analysis of creatinine, uric and ascorbic acid in milk.•The retention loss encountered in reversed-phased HPLC was prevented.•Both creatinine and uric acid were found in bovine milk at significant levels.•Consumption of milk may increase the levels of plasma creatinine and urate.•The HILIC method proved simple, selective, sensitive, accurate and robust. Creatinine (Cr), uric (UA) and ascorbic acid (AA) are common constituents in human fluids. Their abnormal concentrations in human fluids are associated with various diseases. Thus, apart from the endogenous formation in human body, it is also important to examine their sources from food products. In this study, a rapid and accurate HILIC method was developed for simultaneous determination of Cr, UA and AA in bovine milk and orange juice. Milk samples were pretreated by protein precipitation, centrifugation and filtration, followed by HPLC separation and quantification using a Waters Spherisorb S5NH2 column. The developed method has been successfully applied to determine the concentration of UA, AA and Cr in milk and fruit juice samples. The milk samples tested were found to contain UA and creatinine in the concentration range of 24.1–86.0 and 5.07–11.2μgmL−1, respectively. The orange juices contain AA over 212μgmL−1.