Details

Autor(en) / Beteiligte

• Bataglion, Giovana A.
• da Silva, Felipe M.A.
• Eberlin, Marcos N.
• Koolen, Hector H.F.

Titel

Simultaneous quantification of phenolic compounds in buriti fruit (Mauritia flexuosa L.f.) by ultra-high performance liquid chromatography coupled to tandem mass spectrometry

Ist Teil von

• Food research international, 2014-12, Vol.66, p.396-400

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2014

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• A novel analytical approach based on UHPLC–ESI(−)-MS/MS has been performed and evaluated for the identification and quantification of phenolic compounds (phenolic acids and flavonoids/catechins) in buriti (Mauritia flexuosa L.f.) fruit pulp. Due to the selectivity provided by MMR monitoring, UHPLC separation required only 10min per analysis. Linearity (0.994<r2<0.999), limit of detection (8.39–109.80pgmL−1) and limit of quantification (25.42–332.90pgmL−1), inter- and intraday accuracies (>80%) and precisions (CV<20%), and recovery rate (<80%) show that the developed method is reliable to analyze phenolic compounds in fruit extracts. Among the thirteen phenolic compounds analyzed by proposed method, buriti fruit pulp extract showed 6 phenolic acids such as p-coumaric acid, ferulic acid, caffeic acid, protocatechuic acid, chlorogenic acid and quinic acid and 7 flavonoids such as (+)-catechin, (−)-epicatechin, apigenin, luteolin, myricetin, kaempferol and quercetin. As a conclusion, the method was successfully developed and applied representing a contribution to the chemical characterization and quantification of phenolic compounds from buriti fruits which have acknowledged antioxidant and antimicrobial activities. [Display omitted] •A sensitive method for simultaneous quantification of 13 phenolics was developed and validated by UPLC–ESI-MS/MS.•Different quality controls were applied for testing the method.•The method was applied for buriti fruits.